AP3D1 Polyclonal antibody, PBS Only

AP3D1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16454-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HepG2 using 16454-1-AP (same clone as 16454-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16454-1-AP (same clone as 16454-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16454-1-AP (same clone as 16454-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 16454-1-AP (same clone as 16454-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 16454-1-AP (same clone as 16454-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AP3D1 antibody (16454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

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Product Information

16454-1-PBS targets AP3D1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AP3D1 fusion protein Ag9390
• Full Name: adaptor-related protein complex 3, delta 1 subunit
• Calculated Molecular Weight: 1153 aa, 130 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC010065
• Gene Symbol: AP3D1
• Gene ID (NCBI): 8943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3D1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.