AP2A1 Polyclonal antibody

AP2A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30030-1-AP

Synonyms

Alpha adaptin A, alpha Adaptin, ADTAA, Adaptor-related protein complex 2 subunit alpha-1, Adaptor protein complex AP-2 subunit alpha-1

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Validation Data Gallery

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  WB analysis using 30030-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30030-1-AP (AP2A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30030-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30030-1-AP (AP2A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 30030-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30030-1-AP (AP2A1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, MCF-7 cells, SKOV-3 cells, SH-SY5Y cells, mouse kidney tissue, rat kidney tissue, mouse kidney cells, rat kidney cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30030-1-AP targets AP2A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AP2A1 fusion protein Ag32232
• Full Name: adaptor-related protein complex 2, alpha 1 subunit
• Calculated Molecular Weight: 977 aa, 108 kDa
• Observed Molecular Weight: 108 kDa
• GenBank Accession Number: BC014214
• Gene Symbol: AP2A1
• Gene ID (NCBI): 160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.