AP1S3 Polyclonal antibody

• 

  IHC staining of mouse stomach using 17365-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17365-1-AP (AP1S3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 17365-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17365-1-AP (AP1S3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AP1S3 Polyclonal Antibody for IHC, ELISA

Cat No. 17365-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

Sigma1C-adaptin, Sigma 1C subunit of AP-1 clathrin, Golgi adaptor HA1/AP1 adaptin sigma-1C subunit, Clathrin assembly protein complex 1 sigma-1C small chain, Adaptor-related protein complex 1 subunit sigma-1C

---

Tested Applications

• Positive IHC detected in: mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17365-1-AP targets AP1S3 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AP1S3 fusion protein Ag10751
• Full Name: adaptor-related protein complex 1, sigma 3 subunit
• Calculated Molecular Weight: 104aa,11 kDa; 154aa,18 kDa
• GenBank Accession Number: BC021898
• Gene Symbol: AP1S3
• Gene ID (NCBI): 130340
• RRID: AB_3085522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96PC3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP1S3 is a subunit of the adaptor complex AP-1. It belongs to the adaptor complexes small subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.