AOC3 Monoclonal antibody, PBS Only

AOC3 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A6G3

Cat no : 66834-1-PBS

Synonyms

AOC3, Copper amine oxidase, HPAO, Membrane primary amine oxidase, SSAO, VAP 1, VAP1, Vascular adhesion protein 1

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Validation Data Gallery

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  WB analysis of human ileum using 66834-1-Ig (same clone as 66834-1-PBS)

  human ileum tissue were subjected to SDS PAGE followed by western blot with 66834-1-Ig (AOC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using AOC3 antibody (66834-1-Ig, Clone: 2A6G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using AOC3 antibody (66834-1-Ig, Clone: 2A6G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66834-1-PBS targets AOC3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AOC3 fusion protein Ag5908
• Full Name: amine oxidase, copper containing 3 (vascular adhesion protein 1)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: BC050549
• Gene Symbol: AOC3
• Gene ID (NCBI): 8639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

AOC3(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, VAP1 and belongs to the copper/topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions (PMID:17947691). AOC3 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated (PMID:16046623). AOC3 has molecular mass of 70-90kD, and can exsit as a homodimer(165-185kD) and trimer(240~260kD) (PMID:10595925, 8625974).