VAP1 Polyclonal antibody

VAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 14365-1-AP

Synonyms

AOC3, HPAO, EC:1.4.3.21, Copper amine oxidase, Amine oxidase copper-containing 3

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Validation Data Gallery

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  WB analysis using 14365-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14365-1-AP (VAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14365-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14365-1-AP (VAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human appendicitis using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14365-1-AP (VAP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14365-1-AP (VAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14365-1-AP (VAP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse adipose tissue, rat lung tissue, mouse stomach tissue
• Positive IHC detected in: human appendicitis tissue, human lung cancer tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

14365-1-AP targets VAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VAP1 fusion protein Ag5683
• Full Name: amine oxidase, copper containing 3 (vascular adhesion protein 1)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: BC050549
• Gene Symbol: AOC3
• Gene ID (NCBI): 8639
• RRID: AB_2058043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AOC3(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, VAP1 and belongs to the copper/topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions (PMID:17947691). AOC3 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated (PMID:16046623). AOC3 has molecular mass of 70-90kD, and can exsit as a homodimer(165-185kD) and trimer(240~260kD) (PMID:10595925, 8625974).

Publications

Species	Application	Title
human	IHC	J Cell Mol Med; Single-cell transcriptional profiling of human carotid plaques reveals a subpopulation of endothelial cells associated with stroke incidences.; Authors - Fengchan Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (10-21-2020)

Right size band and Clean background, but with relatively weak signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse liver lysate

VAP1 Antibody Western Blot validation (1:1000 dilution) in mouse liver lysate (Cat no:14365-1-AP)