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Annexin A7 Polyclonal antibody
Annexin A7 Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, FC, ELISA
Cat no : 10154-2-AP
Validation Data Gallery
|Positive WB detected in||A549 cells, Jurkat cells, mouse lung tissue, L02 cells, mouse brain tissue, mouse heart tissue, HeLa cells, RAW 264.7 cells, rat brain tissue|
|Positive IP detected in||U-87 MG cells, mouse heart tissue|
|Positive IHC detected in||human pancreas tissue, human prostate cancer tissue, human stomach tissue, human stomach cancer tissue, rat stomach tissue, mouse stomach tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:2000-1:16000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 8 publications below|
|IHC||See 3 publications below|
|IF||See 2 publications below|
10154-2-AP targets Annexin A7 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Annexin A7 fusion protein Ag0206|
|Full Name||annexin A7|
|Calculated molecular weight||50 kDa|
|Observed molecular weight||47 kDa, 51 kDa|
|GenBank accession number||BC002632|
|Gene ID (NCBI)||310|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Annexin A7 (Anx7) belongs to a ubiquitous and relatively abundant family of Ca2+-dependent membrane-binding proteins, which are thought to be involved in multiple aspects of cell biology including membrane trafficking, mediation of cell-matrix interactions and membrane organization within cells. Anx7, migrated as a 50 kDa protein in SDS-PAGE, has been proposed to function in the fusion of vesicles, acting as a Ca++ channel and as Ca++ -activated GTPase, thus inducing Ca++ /GTP-dependent secretory events.
|Product Specific Protocols|
|WB protocol for Annexin A7 antibody 10154-2-AP||Download protocol|
|IHC protocol for Annexin A7 antibody 10154-2-AP||Download protocol|
|IP protocol for Annexin A7 antibody 10154-2-AP||Download protocol|
|FC protocol for Annexin A7 antibody 10154-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.
Am J Physiol Endocrinol Metab
Differential expression of the regulated catecholamine secretory pathway in different hereditary forms of pheochromocytoma.
J Proteome Res
Quantitative Mitochondrial Proteomics Reveals ANXA7 as a Crucial Factor in Mitophagy.
Ezrin as a possible diagnostic and/or prognostic biomarker in mice lymphatic metastatic hepatocellular carcinoma in vivo.
Annexins A1 and A2 are recruited to larger lysosomal injuries independently of ESCRTs to promote repair.
Mol Cell Biochem
Effect of annexin A7 suppression on the apoptosis of gastric cancer cells.