Annexin A7 Polyclonal antibody

• Featured Product
• KD/KO Validated

Annexin A7 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 10154-2-AP

Synonyms

ANXA7, OK/SW-cl.95, ANX7, Annexin-7, Annexin VII

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Validation Data Gallery

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  WB analysis using 10154-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10154-2-AP (Annexin A7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10154-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10154-2-AP (Annexin A7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10154-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10154-2-AP (Annexin VII antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10154-2-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10154-2-AP (Annexin VII antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of U-87 MG using 10154-2-AP

  IP result of anti-Annexin A7 (IP:10154-2-AP, 4ug; Detection:10154-2-AP 1:8000) with U-87 MG cells lysate 1160 ug.

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  IP experiment of mouse heart using 10154-2-AP

  IP result of anti-Annexin A7 (IP:10154-2-AP, 4ug; Detection:10154-2-AP 1:800) with mouse heart tissue lysate 3200ug.

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  IHC staining of human pancreas using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10154-2-AP (Annexin VII antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse heart using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10154-2-AP (Annexin VII antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10154-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10154-2-AP (Annexin VII antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 10154-2-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Annexin A7 (10154-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells, mouse lung tissue, L02 cells, mouse brain tissue, mouse heart tissue, HeLa cells, RAW 264.7 cells, rat brain tissue
• Positive IP detected in: U-87 MG cells, mouse heart tissue
• Positive IHC detected in: mouse heart tissue, human pancreas tissue, human prostate cancer tissue, human stomach tissue, human stomach cancer tissue, mouse stomach tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

10154-2-AP targets Annexin A7 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Annexin A7 fusion protein Ag0206
• Full Name: annexin A7
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 47 kDa, 51 kDa
• GenBank Accession Number: BC002632
• Gene Symbol: Annexin A7
• Gene ID (NCBI): 310
• RRID: AB_2227386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A7 (Anx7) belongs to a ubiquitous and relatively abundant family of Ca2+-dependent membrane-binding proteins, which are thought to be involved in multiple aspects of cell biology including membrane trafficking, mediation of cell-matrix interactions and membrane organization within cells. Anx7, migrated as a 50 kDa protein in SDS-PAGE, has been proposed to function in the fusion of vesicles, acting as a Ca++ channel and as Ca++ -activated GTPase, thus inducing Ca++ /GTP-dependent secretory events.

Publications

Species	Application	Title
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	WB	Am J Physiol Endocrinol Metab; Differential expression of the regulated catecholamine secretory pathway in different hereditary forms of pheochromocytoma.; Authors - Eisenhofer Graeme G
human	WB,IF	J Proteome Res; Quantitative Mitochondrial Proteomics Reveals ANXA7 as a Crucial Factor in Mitophagy.; Authors - Kun Meng ; KD Validated
human	IHC	Am J Transl Res; Downregulation of annexin A7 decreases proliferation, migration, and invasion of gastric cancer cells by reducing matrix metalloproteinase 1 and 9 expression.; Authors - Hu-Fang Yuan
mouse	WB	Biofactors; Ezrin as a possible diagnostic and/or prognostic biomarker in mice lymphatic metastatic hepatocellular carcinoma in vivo.; Authors - Ahmed Musa Hago Bakheet
human	WB	FEBS Lett; Annexins A1 and A2 are recruited to larger lysosomal injuries independently of ESCRTs to promote repair.; Authors - Willa Wen-You Yim