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  • KD/KO Validated

Annexin A7 Polyclonal antibody

Annexin A7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10154-2-AP

Synonyms

Annexin 7, annexin A7, Annexin VII, Annexin7, Annexin-7, ANX7, ANXA7, SNX, SYNEXIN



Tested Applications

Positive WB detected inA549 cells, Jurkat cells, mouse lung tissue, L02 cells, mouse brain tissue, mouse heart tissue, HeLa cells, RAW 264.7 cells, rat brain tissue
Positive IP detected inU-87 MG cells, mouse heart tissue, RAW 264.7 cells
Positive IHC detected inhuman pancreas tissue, human prostate cancer tissue, human stomach tissue, human stomach cancer tissue, rat stomach tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10154-2-AP targets Annexin A7 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin A7 fusion protein Ag0206
Full Name annexin A7
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 47 kDa, 51 kDa
GenBank Accession NumberBC002632
Gene Symbol Annexin A7
Gene ID (NCBI) 310
RRIDAB_2227386
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A7 (Anx7) belongs to a ubiquitous and relatively abundant family of Ca2+-dependent membrane-binding proteins, which are thought to be involved in multiple aspects of cell biology including membrane trafficking, mediation of cell-matrix interactions and membrane organization within cells. Anx7, migrated as a 50 kDa protein in SDS-PAGE, has been proposed to function in the fusion of vesicles, acting as a Ca++ channel and as Ca++ -activated GTPase, thus inducing Ca++ /GTP-dependent secretory events.

Protocols

Product Specific Protocols
WB protocol for Annexin A7 antibody 10154-2-APDownload protocol
IHC protocol for Annexin A7 antibody 10154-2-APDownload protocol
IP protocol for Annexin A7 antibody 10154-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Matrix Biol

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Authors - Emna Ouni
humanWB

Am J Physiol Endocrinol Metab

Differential expression of the regulated catecholamine secretory pathway in different hereditary forms of pheochromocytoma.

Authors - Eisenhofer Graeme G
humanWB,IF

J Proteome Res

Quantitative Mitochondrial Proteomics Reveals ANXA7 as a Crucial Factor in Mitophagy.

Authors - Kun Meng
  • KD Validated
humanIHC

Am J Transl Res

Downregulation of annexin A7 decreases proliferation, migration, and invasion of gastric cancer cells by reducing matrix metalloproteinase 1 and 9 expression.

Authors - Hu-Fang Yuan
mouseWB

Biofactors

Ezrin as a possible diagnostic and/or prognostic biomarker in mice lymphatic metastatic hepatocellular carcinoma in vivo.

Authors - Ahmed Musa Hago Bakheet
humanWB

FEBS Lett

Annexins A1 and A2 are recruited to larger lysosomal injuries independently of ESCRTs to promote repair.

Authors - Willa Wen-You Yim