ANXA5 Recombinant antibody

ANXA5 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240621B10

Cat no : 83929-2-RR

Synonyms

Annexin A5, Annexin-5, Annexin V, Annexin 5, Anchorin CII

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Validation Data Gallery

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  WB analysis using 83929-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83929-2-RR (ANXA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83929-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ANXA5 antibody (83929-2-RR, Clone: 240621B10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of PC-3 using 83929-2-RR

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug Anxa5 Recombinant Antibody (83929-2-RR, Clone:240621B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HeLa using 83929-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anxa5 Recombinant Antibody (83929-2-RR, Clone:240621B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83929-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83929-2-RR against Human ANXA5 were performed. The affinity constant is 18.2 nM.

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Tested Applications

• Positive WB detected in: C6 cells, HeLa cells, K-562 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: PC-3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83929-2-RR is ANXA5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ANXA5 fusion protein Ag0741
• Full Name: annexin A5
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 32-36 kDa
• GenBank Accession Number: BC001429
• Gene Symbol: Annexin V
• Gene ID (NCBI): 308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.