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  • KD/KO Validated

Annexin V Polyclonal antibody

Annexin V Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, pig, rat





Cat no : 11060-1-AP


Anchorin CII, Annexin 5, annexin A5, Annexin V, ANX5, ANXA5, Calphobindin I, CBP I, Endonexin II, ENX2, Lipocortin V, PAP I, PP4, Thromboplastin inhibitor, VAC alpha, Vascular anticoagulant alpha

Tested Applications

Positive WB detected inHeLa cells, pig heart tissue, pig liver tissue, human lung tissue, human heart tissue, human placenta tissue, human skeletal muscle tissue, HEK-293 cells, A431 cells, HepG2 cells, NIH/3T3 cells, K-562 cells, NIH?3T3 cells, C2C12 cells, C6 cells
Positive IP detected inHeLa cells, mouse liver tissue
Positive IHC detected inhuman spleen tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11060-1-AP targets Annexin V in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin V fusion protein Ag1538
Full Name annexin A5
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC018671
Gene Symbol ANXA5
Gene ID (NCBI) 308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.


Product Specific Protocols
WB protocol for Annexin V antibody 11060-1-APDownload protocol
IHC protocol for Annexin V antibody 11060-1-APDownload protocol
IF protocol for Annexin V antibody 11060-1-APDownload protocol
IP protocol for Annexin V antibody 11060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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