Annexin A3 Polyclonal antibody

• Featured Product
• KD/KO Validated

Annexin A3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 11804-1-AP

Synonyms

35 alpha calcimedin, Annexin 3, annexin A3, Annexin III, ANX3, ANXA3, Lipocortin III, PAP III

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Validation Data Gallery

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  WB analysis using 11804-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11804-1-AP (Annexin A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11804-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11804-1-AP (Annexin A3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11804-1-AP

  IP result of anti-Annexin A3 (IP:11804-1-AP, 4ug; Detection:11804-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human stomach cancer using 11804-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11804-1-AP (Annexin A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11804-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11804-1-AP (Annexin A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11804-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11804-1-AP (Annexin A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11804-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11804-1-AP (Annexin A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse lung tissue, mouse testis tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human stomach cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• FC: See 1 publications below

Product Information

11804-1-AP targets Annexin A3 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Annexin A3 fusion protein Ag2381
• Full Name: annexin A3
• Calculated Molecular Weight: 322 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC000871
• Gene Symbol: Annexin A3
• Gene ID (NCBI): 306
• RRID: AB_2057455
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.

Publications

Species	Application	Title
mouse	FC	Cancer Immunol Res; ANXA3-rich exosomes derived from tumor-associated macrophages regulate ferroptosis and lymphatic metastasis of laryngeal squamous cell carcinoma; Authors - Licheng Xu
human	IHC	Front Immunol; Upregulated of ANXA3, SORL1, and Neutrophils May Be Key Factors in the Progressionof Ankylosing Spondylitis.; Authors - Jie Jiang
rat	IF	iScience; Deciphering the dynamic niches and regeneration-associated transcriptional program of motoneurons following peripheral nerve injury; Authors - Yu Zhang
mouse	IF	Sci Rep; Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers.; Authors - R Sartaj
mouse	IHC	Front Med (Lausanne); An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.; Authors - Songyan Gao
mouse	WB,IHC	J Proteomics; Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection.; Authors - Fang Jiao