CD13 Polyclonal antibody

CD13 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14553-1-AP


Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN

Tested Applications

Positive WB detected inmouse kidney tissue, human kidney tissue, human liver tissue
Positive IHC detected inhuman tonsillitis tissue, human colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse kidney tissue
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CD13 Ready-To-Use IHC Kit. CD13 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14553-1-AP targets CD13 in WB, IHC, IF, FC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD13 fusion protein Ag5976
Full Name alanyl (membrane) aminopeptidase
Calculated molecular weight 110 kDa
Observed molecular weight 150 kDa, 135 kDa, 110 kDa
GenBank accession numberBC058928
Gene symbol ANPEP
Gene ID (NCBI) 290
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)


Product Specific Protocols
WB protocol for CD13 antibody 14553-1-APDownload protocol
IHC protocol for CD13 antibody 14553-1-APDownload protocol
IF protocol for CD13 antibody 14553-1-APDownload protocol
FC protocol for CD13 antibody 14553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Anal Chem

Rapid Screening of Peptide Probes through in situ Single Bead Sequencing Microarray.

Authors - Weizhi Wang

PLoS Negl Trop Dis

Proteomic Analysis of Urine Exosomes Reveals Renal Tubule Response to Leptospiral Colonization in Experimentally Infected Rats.

Authors - Satish P RamachandraRao

Neuropathol Appl Neurobiol

Brain mural cell loss in the parietal cortex in Alzheimer's disease correlates with cognitive decline and TDP-43 pathology.

Authors - P Bourassa

Mol Cancer

Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.

Authors - Yang Gu