CD13 Polyclonal antibody

CD13 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 14553-1-AP

Synonyms

APN, AP-M, ANPEP, Aminopeptidase N, Aminopeptidase M

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Validation Data Gallery

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  WB analysis using 14553-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14553-1-AP (CD13 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14553-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14553-1-AP (CD13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14553-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14553-1-AP (CD13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14553-1-AP (CD13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14553-1-AP (CD13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14553-1-AP (CD13 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14553-1-AP (CD13 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14553-1-AP (CD13 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14553-1-AP (CD13 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse kidney using 14553-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 14553-1-AP (CD13 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 14553-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CD13 antibody (14553-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HepG2 cells, human liver tissue, human kidney tissue, PC-3 cells, THP-1 cells, mouse kidney tissue, mouse liver tissue
• Positive IHC detected in: human tonsillitis tissue, human colon tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

14553-1-AP targets CD13 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, canine, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD13 fusion protein Ag5976
• Full Name: alanyl (membrane) aminopeptidase
• Calculated Molecular Weight: 110 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC058928
• Gene Symbol: CD13
• Gene ID (NCBI): 290
• RRID: AB_2056591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)

Publications

Species	Application	Title
human	WB	Mol Cancer; Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.; Authors - Yang Gu
human		Anal Chem; Rapid Screening of Peptide Probes through in situ Single Bead Sequencing Microarray.; Authors - Weizhi Wang
human	WB,IF	Neuropathol Appl Neurobiol; Brain mural cell loss in the parietal cortex in Alzheimer's disease correlates with cognitive decline and TDP-43 pathology.; Authors - P Bourassa
human	WB	Front Oncol; YBX1 Enhances Metastasis and Stemness by Transcriptionally Regulating MUC1 in Lung Adenocarcinoma.; Authors - Qiang Xie
rat	WB	PLoS Negl Trop Dis; Proteomic Analysis of Urine Exosomes Reveals Renal Tubule Response to Leptospiral Colonization in Experimentally Infected Rats.; Authors - Satish P RamachandraRao
mouse	IF	Exp Cell Res; siRNA knockdown of alanine aminopeptidase impairs myoblast proliferation and differentiation.; Authors - Shion Osana