ANP32B Monoclonal antibody, PBS Only (Capture)

• Featured Product
• KD/KO Validated

ANP32B Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5A1F12

Cat no : 66160-1-PBS

Synonyms

Silver-stainable protein SSP29, Putative HLA-DR-associated protein I-2, PHAPI2, APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B

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Validation Data Gallery

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  WB analysis using 66160-1-Ig (same clone as 66160-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66160-1-Ig (ANP32B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66160-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66160-1-Ig (same clone as 66160-1-PBS)

  WB result of ANP32B antibody (66160-1-Ig, 1:5000) with si-control and si-ANP32B transfected HEK293 cells. This data was developed using the same antibody clone with 66160-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66160-1-Ig (same clone as 66160-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66160-1-Ig (ANP32B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66160-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66160-1-Ig (same clone as 66160-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66160-1-Ig (ANP32B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66160-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66160-1-Ig (same clone as 66160-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32B antibody (66160-1-Ig, Clone: 5A1F12 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66160-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50363-1

  Cytometric bead array standard curve of MP50363-1, ANP32B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66160-1-PBS. Detection antibody: 66160-2-PBS. Standard:Ag1274. Range: 1.563-200 ng/mL.

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  Cytometric bead array standard curve of MP50363-2

  Cytometric bead array standard curve of MP50363-2, ANP32B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66160-1-PBS. Detection antibody: 66160-3-PBS. Standard:Ag1274. Range: 1.563-200 ng/mL.

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Product Information

66160-1-PBS targets ANP32B as part of a matched antibody pair:

MP50363-1: 66160-1-PBS capture and 66160-2-PBS detection (validated in Cytometric bead array)

MP50363-2: 66160-1-PBS capture and 66160-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ANP32B fusion protein Ag1274
• Full Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC013003
• Gene Symbol: ANP32B
• Gene ID (NCBI): 10541
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).