ANP32A Monoclonal antibody, PBS Only

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  WB analysis using 67687-1-Ig (same clone as 67687-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67687-1-Ig (ANP32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67687-1-Ig (same clone as 67687-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67687-1-Ig (ANP32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32A antibody (67687-1-Ig, Clone: 2D8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32A antibody (67687-1-Ig, Clone: 2D8C5 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

ANP32A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67687-1-PBS

Clone No.2D8C5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1, Acidic leucine-rich nuclear phosphoprotein 32 family member A

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Product Information

67687-1-PBS targets ANP32A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ANP32A fusion protein Ag8546
• Full Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
• Calculated Molecular Weight: 249 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC007200
• Gene Symbol: ANP32A
• Gene ID (NCBI): 8125
• RRID: AB_2882880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P39687
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.