TMEM16A/DOG1 Recombinant antibody, PBS Only (Detector)

TMEM16A/DOG1 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241464C11

Cat no : 83567-3-PBS

Synonyms

ANO1, 241464C11, DOG1, Discovered on gastrointestinal stromal tumors protein 1, Anoctamin-1

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01115-3

  Sandwich ELISA standard curve of MP01115-3, Human TMEM16A/DOG1 Recombinant Matched Antibody Pair - PBS only. 83567-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3320. 83567-3-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

• 

  Sample test of MP01115-3

  The mean TMEM16A/DOG1 concentration was determined to be 16.1 ng/mL in HeLa cell extract based on a 1.3 mg/mL extract load.

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  Cytometric bead array standard curve of MP01115-1

  Cytometric bead array standard curve of MP01115-1, ANO1/TMEM16A/DOG1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83567-5-PBS. Detection antibody: 83567-3-PBS. Standard: Ag3320. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83567-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83567-3-PBS against Human TMEM16A/DOG1 was performed. The affinity constant is below 1 pM.

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Product Information

83567-3-PBS targets TMEM16A/DOG1 as part of a matched antibody pair:

MP01115-1: 83567-5-PBS capture and 83567-3-PBS detection (validated in Cytometric bead array)

MP01115-3: 83567-7-PBS capture and 83567-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TMEM16A/DOG1 fusion protein Ag3320
• Full Name: anoctamin 1, calcium activated chloride channel
• Calculated Molecular Weight: 986 aa, 114 kDa
• GenBank Accession Number: BC027590
• Gene Symbol: TMEM16A
• Gene ID (NCBI): 55107
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.