ANKS1B Polyclonal antibody

ANKS1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24783-1-AP

Synonyms

AIDA, AIDA 1, ANKS1B, ANKS2, cajalin 2, E2A PBX1 associated protein, EB 1, EB1

---

Validation Data Gallery

• 

  WB analysis of A549 using 24783-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 24783-1-AP (ANKS1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24783-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24783-1-AP (ANKS1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 24783-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24783-1-AP (ANKS1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 24783-1-AP

  IP result of anti-ANKS1B (IP:24783-1-AP, 4ug; Detection:24783-1-AP 1:1000) with HeLa cells lysate 3200ug.

• 

  IHC staining of mouse brain using 24783-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24783-1-AP (ANKS1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 24783-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24783-1-AP (ANKS1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 24783-1-AP

  Immunofluorescent analysis of HepG2 cells using 24783-1-AP (ANKS1B antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

---

Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, mouse testis tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 24783-1-AP is ANKS1B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANKS1B fusion protein Ag20454
• Full Name: ankyrin repeat and sterile alpha motif domain containing 1B
• Calculated Molecular Weight: 1248 aa, 138 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC026313
• Gene Symbol: ANKS1B
• Gene ID (NCBI): 56899
• RRID: AB_2879720
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKS1B also named as AIDA or cajalin 2 is a amino acid protein, which contains 7 ANK repeats, 1 PID domain and 2 SAM domains. ANKS1B localizes in cytoplasm and is highly expressed in brain and testis. ANKS1B exists as many alternatively spliced isoforms. It interacts with AbPP to play a role in brain development. AIDA-1 also interacts with coilin in Cajal bodies to regulate pre-mRNA splicing.

Publications

Species	Application	Title
human	WB	EBioMedicine; Juvenile CLN3 disease is a lysosomal cholesterol storage disorder: similarities with Niemann-Pick type C disease; Authors - Jacinda Chen