ANKRD55 Polyclonal antibody

ANKRD55 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24203-1-AP

Synonyms

ANKRD55, ankyrin repeat domain 55

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Validation Data Gallery

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  WB analysis of HeLa using 24203-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24203-1-AP (ANKRD55 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 24203-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 24203-1-AP (ANKRD55 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 24203-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24203-1-AP (ANKRD55 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 24203-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24203-1-AP (ANKRD55 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 24203-1-AP

  Immunofluorescent analysis of HeLa cells using 24203-1-AP (ANKRD55 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, L02 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24203-1-AP is ANKRD55 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANKRD55 fusion protein Ag21271
• Full Name: ankyrin repeat domain 55
• Calculated Molecular Weight: 614 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC106914
• Gene Symbol: ANKRD55
• Gene ID (NCBI): 79722
• RRID: AB_2879456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKRD55 also named as Ankyrin repeat domain containing protein 55 is a 614 amino acid protein, which contains 9 ANK repeat. ANKRD55 may exist three alternatively spliced isoforms and belongs to ankyrin family. The function of ANKRD55 is still non-known and need to be further studied.