ANKRD49 Polyclonal antibody

ANKRD49 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25034-1-AP

Synonyms

ANKRD49, ankyrin repeat domain 49, Fetal globin inducing factor, FGIF, GBIF

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Validation Data Gallery

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  WB analysis using 25034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25034-1-AP (ANKRD49 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

25034-1-AP targets ANKRD49 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANKRD49 fusion protein Ag18295
• Full Name: ankyrin repeat domain 49
• Calculated Molecular Weight: 239 aa, 27 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC017798
• Gene Symbol: ANKRD49
• Gene ID (NCBI): 54851
• RRID: AB_2879860
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ankyrin repeat domain-containing protein 49 (ANKRD49), an evolutionarily conserved protein highly expressed in the testes, is mainly found in spermatogonia, spermatocytes, and round spermatids. ANKRD49 also participates in the progression of malignant gliomas and gastric cancer in humans. (PMID: 37964204, PMID: 30798416)

Publications

Species	Application	Title
human	WB,IHC	J Cell Mol Med; ANKRD49 promotes the invasion and metastasis of lung adenocarcinoma via a P38/ATF-2 signalling pathway.; Authors - Yue-Hua Liu
human	WB	Appl Immunohistochem Mol Morphol; A Microarray Study on the Expression of ANKRD49 in Lung Squamous Cell Carcinoma and Its Clinicopathologic Significance.; Authors - Xin-Yang Li
human,mouse	WB,IHC	BMC Cancer; ANKRD49 promotes the metastasis of NSCLC via activating JNK-ATF2/c-Jun-MMP-2/9 axis; Authors - Jia Sun