ANKRD2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ANKRD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11821-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11821-1-AP (ANKRD2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11821-1-AP (ANKRD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

  IP result of anti-ANKRD2 (IP:11821-1-AP, 4ug; Detection:11821-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 11821-1-AP (same clone as 11821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 11821-1-AP (same clone as 11821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 11821-1-AP (same clone as 11821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 11821-1-AP (same clone as 11821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 11821-1-AP (same clone as 11821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 11821-1-AP (same clone as 11821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 11821-1-AP (same clone as 11821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 11821-1-AP (same clone as 11821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 11821-1-AP (same clone as 11821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11821-1-AP (ANKRD2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 11821-1-AP (same clone as 11821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11821-1-AP (ANKRD2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11821-1-AP (same clone as 11821-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11821-1-AP (ANKRD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

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Product Information

11821-1-PBS targets ANKRD2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANKRD2 fusion protein Ag2389
• Full Name: ankyrin repeat domain 2 (stretch responsive muscle)
• Calculated Molecular Weight: 37 aa, 2 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC020817
• Gene Symbol: ANKRD2
• Gene ID (NCBI): 26287
• RRID: AB_2057858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ANKRD2 belongs to the conserved muscle ankyrin repeat protein (MARP) family. Expression of MARPs is induced in response to physiologic stress, injury, and hypertrophy. Together with Ankrd1/CARP and DARP, ANKRD2 are members of the MARP mechanosensing proteins that form a complex with titin (N2A)/calpain 3 protease/myopalladin. Ankrd2 is thought to have dual, structural and signaling roles, and could link the elastic I-band region as a stress sensor for transcriptional control in the nucleus