ANKRD16 Polyclonal antibody

ANKRD16 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24334-1-AP

Synonyms

Ankyrin repeat domain-containing protein 16, ankyrin repeat domain 16

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Validation Data Gallery

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  WB analysis of HeLa using 24334-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24334-1-AP (ANKRD16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 24334-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24334-1-AP (ANKRD16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 24334-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24334-1-AP (ANKRD16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24334-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24334-1-AP (ANKRD16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 24334-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 24334-1-AP (ANKRD16 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 24334-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ANKRD16 antibody (24334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, PC-3 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24334-1-AP targets ANKRD16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANKRD16 fusion protein Ag19369
• Full Name: ankyrin repeat domain 16
• Calculated Molecular Weight: 361 aa, 39 kDa
• Observed Molecular Weight: 39-45 kDa
• GenBank Accession Number: BC062346
• Gene Symbol: ANKRD16
• Gene ID (NCBI): 54522
• RRID: AB_2879499
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKRD16, a vertebrate-specific protein that contains ankyrin repeats, binds directly to the catalytic domain of AlaRS. Serine that is misactivated by AlaRS is captured by the lysine side chains of ANKRD16, which prevents the charging of serine adenylates to tRNAAla and precludes serine misincorporation in nascent peptides. The deletion of Ankrd16 in the brains of Aarssti/sti mice causes widespread protein aggregation and neuron loss (PMID: 29769718).