ANKRD1 Polyclonal antibody

ANKRD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

15

Reviews (1)

Cat no : 11427-1-AP

Synonyms

ALRP, ANKRD1, C 193, C193, Cardiac ankyrin repeat protein, CARP, CVARP, HA1A2, MCARP

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Validation Data Gallery

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  WB analysis using 11427-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11427-1-AP (ANKRD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 11427-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11427-1-AP (ANKRD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

11427-1-AP targets ANKRD1 in WB, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANKRD1 fusion protein Ag1970
• Full Name: ankyrin repeat domain 1 (cardiac muscle)
• Calculated Molecular Weight: 319 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC018667
• Gene Symbol: ANKRD1
• Gene ID (NCBI): 27063
• RRID: AB_2227402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression. This is a rabbit polyclonal antibody produced by the full-length ANKRD1 of human origin

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Metabolic control of YAP and TAZ by the mevalonate pathway.; Authors - Giovanni Sorrentino
mouse	IF	Nat Commun; YAP1/TAZ drives ependymoma-like tumour formation in mice.; Authors - Noreen Eder
human	WB	Nat Commun; ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.; Authors - Rokana Taftaf
human	WB	Nat Commun; Glucocorticoid receptor signalling activates YAP in breast cancer.; Authors - Giovanni Sorrentino
		Hum Mol Genet; Exploiting The CRISPR/CAS9 System to Study Alternative Splicing In Vivo: Application to Titin.; Authors - Karine Charton
mouse	IF	Cell Death Dis; The histone code reader Spin1 controls skeletal muscle development.; Authors - Holger Greschik

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Luigi (Verified Customer) (11-29-2018)

• Applications: Immunoprecipitation,
• Primary Antibody Dilution: 5 ug
• Cell Tissue Type: Fibroblast

ANKRD1 Antibody Immunoprecipitation, validation (5 ug dilution) in Fibroblast (Cat no:11427-1-AP)