ANGPTL4 Monoclonal antibody, PBS Only

ANGPTL4 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D2H6

Cat no : 67577-1-PBS

Synonyms

angiopoietin like 4, Angiopoietin like protein 4, Angiopoietin related protein 4, ANGPTL4, ARP4, FIAF, HFARP, NL2, PGAR, pp1158, PSEC0166, UNQ171/PRO197

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Validation Data Gallery

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  WB analysis of human placenta using 67577-1-Ig (same clone as 67577-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67577-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 67577-1-Ig (same clone as 67577-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67577-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 67577-1-Ig (same clone as 67577-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67577-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 67577-1-Ig (same clone as 67577-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67577-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Caco-2 using 67577-1-Ig (same clone as 67577-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67577-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 67577-1-Ig (same clone as 67577-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67577-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67577-1-Ig (same clone as 67577-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67577-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 67577-1-Ig (same clone as 67577-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ANGPTL4 antibody (67577-1-Ig, Clone: 1D2H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67577-1-PBS in a different storage buffer formulation.

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Product Information

67577-1-PBS targets ANGPTL4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ANGPTL4 fusion protein Ag30070
• Full Name: angiopoietin-like 4
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC023647
• Gene Symbol: ANGPTL4
• Gene ID (NCBI): 51129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three isoforms, 45 kDa, 41 kDa and 27 kDa.