ANAPC5 Polyclonal antibody

ANAPC5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14926-1-AP

Synonyms

ANAPC5, APC5, Cyclosome subunit 5

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Validation Data Gallery

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  WB analysis of mouse lung using 14926-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14926-1-AP (ANAPC5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 14926-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14926-1-AP (ANAPC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 14926-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14926-1-AP (ANAPC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 14926-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14926-1-AP (ANAPC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14926-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14926-1-AP (ANAPC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue, BxPC-3 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14926-1-AP targets ANAPC5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANAPC5 fusion protein Ag6688
• Full Name: anaphase promoting complex subunit 5
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC001081
• Gene Symbol: ANAPC5
• Gene ID (NCBI): 51433
• RRID: AB_2227073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.