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AMY2B Monoclonal antibody

AMY2B Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E5G2

Cat no : 68474-1-Ig

Synonyms

Alpha amylase 2B, AMY2, AMY2B, amylase, alpha 2B (pancreatic), Carcinoid alpha amylase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMY2B Monoclonal antibody (68474-1-Ig)

    WB analysis using 68474-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68474-1-Ig (AMY2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inpig pancreas tissue, rat pancreas tissue，mouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68474-1-Ig targets AMY2B in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AMY2B fusion protein Ag3260
Full Name amylase, alpha 2B (pancreatic)
Calculated Molecular Weight 511 aa, 58 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC011179
Gene Symbol AMY2B
Gene ID (NCBI) 280
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMY2B(alpha-amylase 2B) belongs to the glycosyl hydrolase 13 family. This protein is involved in the endohydrolysis of 1,4-alpha-glucosidic linkages in oligosaccharides and polysaccharides. Human amylases (Amy1, Amy2A, and Amy2B) commonly have two potential N-glycosylation sites (N427 and N476) in their C-terminal region (S Takashima, J Amano, 2012).

Protocols

Product Specific Protocols
WB protocol for AMY2B antibody 68474-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols