Amylase Alpha Polyclonal antibody

Amylase Alpha Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15845-1-AP

Synonyms

AMY2A, Amylase, alpha, Pancreatic alpha amylase

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Validation Data Gallery

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  WB analysis of human liver using 15845-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15845-1-AP (Amylase, alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 15845-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15845-1-AP (Amylase, alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 15845-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15845-1-AP (Amylase, alpha antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 15845-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15845-1-AP (Amylase, alpha antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human liver tissue, mouse pancreas tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

15845-1-AP targets Amylase Alpha in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Amylase Alpha fusion protein Ag8605
• Full Name: amylase, alpha 2A (pancreatic)
• Calculated Molecular Weight: 511 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC007060
• Gene Symbol: Amylase Alpha
• Gene ID (NCBI): 279
• RRID: AB_2242664
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The complete human amylase gene contains five intact genes: two pancreatic genes (AMY2A and AMY2B) and three salivary genes (AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258).AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956). 15845-1-AP can recognize both AMY1 and AMY2.

Publications

Species	Application	Title
human	IHC	J Toxicol Pathol; Databases for technical aspects of immunohistochemistry: 2021 update.; Authors - Takayasu Moroki
	IHC	J Toxicol Pathol; Databases for technical aspects of immunohistochemistry.; Authors - Satoshi Furukawa