AMT Monoclonal antibody, PBS Only (Detector)

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  WB analysis of pig liver using 67532-1-Ig (same clone as 67532-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 67532-1-Ig (same clone as 67532-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 67532-1-Ig (same clone as 67532-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67532-1-Ig (same clone as 67532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67532-1-Ig (same clone as 67532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67532-1-Ig (same clone as 67532-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AMT antibody (67532-1-Ig, Clone: 3E7D4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50403-2

  Cytometric bead array standard curve of MP50403-2, AMT Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67532-3-PBS. Detection antibody: 67532-1-PBS. Standard:Ag26644. Range: 3.125-100 ng/mL.

AMT Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 67532-1-PBS

Clone No.3E7D4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

GCST, GCVT, EC:2.1.2.10, aminomethyltransferase, 3E7D4

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Product Information

67532-1-PBS targets AMT as part of a matched antibody pair:

MP50403-2: 67532-3-PBS capture and 67532-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AMT fusion protein Ag26644
• Full Name: aminomethyltransferase
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 37-44 kDa
• GenBank Accession Number: BC007546
• Gene Symbol: AMT
• Gene ID (NCBI): 275
• RRID: AB_2882751
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P48728
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.