AMPD1-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

AMPD1-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 19780-1-AP

Synonyms

AMP deaminase 1, AMP deaminase isoform M, AMPD1, AMPD1-Specific, MAD, MADA, Myoadenylate deaminase

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 19780-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 19780-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 19780-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:300.

• 

  IHC staining of mouse skeletal muscle using 19780-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 19780-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

19780-1-AP targets AMPD1-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: adenosine monophosphate deaminase 1 (isoform M)
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 75-83 kDa
• GenBank Accession Number: NM_000036
• Gene Symbol: AMPD1
• Gene ID (NCBI): 270
• RRID: AB_10644281
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMPD1, also named as MAD and MADA, belongs to the adenosine and AMP deaminases family. AMP deaminase plays a critical role in energy metabolism. It catalyzes the reaction: AMP + H2O = IMP + NH3. Defects in AMPD1 are the cause of adenosine monophosphate deaminase deficiency muscle type (AMPDDM) which is a metabolic disorder resulting in exercise-related myopathy. The antibody is specific to AMPD1.

Publications

Species	Application	Title
mouse	WB	iScience; Phosphate depletion in insulin-insensitive skeletal muscle drives AMPD activation and sarcopenia in chronic kidney disease; Authors - Ana Andres-Hernando ; KO Validated
human	IHC	Front Oncol; AMPD1 Is Associated With the Immune Response and Serves as a Prognostic Marker in HER2-Positive Breast Cancer.; Authors - Long Wang
mouse	WB	Sci Adv; Lactate metabolism is essential in early-onset mitochondrial myopathy; Authors - Zhenkang Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Laila (Verified Customer) (07-22-2022)

This antibody worked great with Bovine tissue with a total of 60 ug of protein and very little non-specific binding.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: bovine skeletal muscle

AMPD1-Specific Antibody Western Blot validation (1:1000 dilution) in bovine skeletal muscle (Cat no:19780-1-AP)