AMOT Monoclonal antibody, PBS Only

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  WB analysis of SGC-7901 using 60156-1-Ig (same clone as 60156-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60156-1-Ig (AMOT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60156-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60156-1-Ig (same clone as 60156-1-PBS)

  WB result of AMOT antibody (60156-1-Ig, 1:1000) with si-Control and si-AMOT transfected HEK293 cells. This data was developed using the same antibody clone with 60156-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 60156-1-Ig (same clone as 60156-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using AMOT antibody (60156-1-Ig, Clone: 3H7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60156-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

AMOT Monoclonal Antibody for WB, IF-P, Indirect ELISA

Cat No. 60156-1-PBS

Clone No.3H7B3

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF-P, Indirect ELISA

Synonyms

KIAA1071, Angiomotin, 3H7B3

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Product Information

60156-1-PBS targets AMOT in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: peptide
• Full Name: angiomotin
• Calculated Molecular Weight: 118 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: NM_001113490
• Gene Symbol: AMOT
• Gene ID (NCBI): 154796
• RRID: AB_10644279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q4VCS5
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. The most abundant expression was found in the placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). This antibody only recognizes p130.