AMN Polyclonal antibody

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  WB analysis using 32038-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32038-1-AP (AMN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 32038-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AMN antibody (32038-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

AMN Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 32038-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Protein amnionless, PRO1028, IGS2, Amnionless Homolog, Amnionless

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Tested Applications

• Positive WB detected in: HuH-7 cells, Jurkat cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32038-1-AP targets AMN in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMN fusion protein Ag35205
• Full Name: amnionless homolog (mouse)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: NM_030943.3
• Gene Symbol: AMN
• Gene ID (NCBI): 81693
• RRID: AB_3670177
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9BXJ7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Amnion associated transmembrane protein (AMN, also known as IGS2, PRO1028, and amnionless) is a 45-50 kDa cysteine-rich type I transmembrane protein that is expressed exclusively in the extra-embryonic visceral endoderm layer during gastrulation (PMID: 17990981; 11279523). It may direct the production of trunk mesoderm derived from the middle streak by acting in the underlying visceral endoderm to modulate a BMP signaling pathway (PMID: 11279523).