JAML Polyclonal antibody

JAML Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21302-1-AP

Synonyms

AMICA, AMICA1, CREA7 1, CREA7 4, Gm638, JAML



Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells, L02 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21302-1-AP targets JAML in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JAML fusion protein Ag15900
Full Name adhesion molecule, interacts with CXADR antigen 1
Calculated Molecular Weight 394 aa, 44 kDa
Observed Molecular Weight 44 kDa, 50-70 kDa
GenBank Accession NumberBC100797
Gene Symbol JAML
Gene ID (NCBI) 120425
RRIDAB_10733103
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Junctional adhesion molecules (JAM-A, JAM-B, and JAM-C) are endothelial and epithelial adhesion molecules involved in the recruitment of circulating leukocytes to inflammatory sites (PMID: 19064666). JAML (junctional adhesion molecule-like), also known as AMICA1, is related to JAM proteins. It contains 2 extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail. JAML is expressed on leukocytes and promotes their adhesion to endothelial cells (PMID: 12869515). JAML can regulate neutrophil transepithelial migration via binding interactions with epithelial coxsackie and adenovirus receptor (CAR) (PMID: 15800062). The full-length human JAML protein has a calculated molecular weight of 44 kDa. This antibody raised against 40-275 aa of human JAML protein detects bands with molecular wight of 44 kDa, and 50-70 kDa which probably represent different glycoforms of the protein (PMID: 12869515; 24621992; 15800062).

Protocols

Product Specific Protocols
WB protocol for JAML antibody 21302-1-APDownload protocol
IHC protocol for JAML antibody 21302-1-APDownload protocol
IF protocol for JAML antibody 21302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

J Transl Med

Junctional adhesion molecule-like protein promotes tumor progression via the Wnt/β-catenin signaling pathway in lung adenocarcinoma.

Authors - Qian Wu
mouseWB,IHC

Eur J Pharmacol

Resveratrol ameliorates diabetic kidney injury by reducing lipotoxicity and modulates expression of components of the junctional adhesion molecule-like/sirtuin 1 lipid metabolism pathway.

Authors - Wei Gu
mouseIHC,IF

Clin Sci (Lond)

Silencing of junctional adhesion molecule-like protein attenuates atherogenesis and enhances plaque stability in ApoE-/- mice.

Authors - Yu Sun