AMD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

AMD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 11052-1-AP

Synonyms

ADOMETDC, AMD, AMD1, SAMDC

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Validation Data Gallery

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  WB analysis of A431 using 11052-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11052-1-AP (AMD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 11052-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11052-1-AP (AMD1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 11052-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11052-1-AP (AMD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 11052-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11052-1-AP (AMD1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IHC detected in: human prostate cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

11052-1-AP targets AMD1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMD1 fusion protein Ag1525
• Full Name: adenosylmethionine decarboxylase 1
• Calculated Molecular Weight: 38 kDa, 32 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC000171
• Gene Symbol: AMD1
• Gene ID (NCBI): 262
• RRID: AB_2226430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Nature; mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer.; Authors - Amaia Zabala-Letona ; KD Validated
human	WB,IF	Nucleic Acids Res; Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism.; Authors - Kerstin Dörner
human	WB, IHC	Clin Transl Med; AMD1 upregulates hepatocellular carcinoma cells stemness by FTO mediated mRNA demethylation.; Authors - Xinyu Bian ; KD Validated
human	WB,IF	Breast Cancer Res; AMD1 promotes breast cancer aggressiveness via a spermidine-eIF5A hypusination-TCF4 axis; Authors - Ruocen Liao
human	WB	iScience; The KRAS-G12D mutation induces metabolic vulnerability in B-cell acute lymphoblastic leukemia.; Authors - Yan Xu
human	WB	iScience; Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.; Authors - Jun Yamamoto