AMACR/p504S Recombinant antibody

• 

  WB analysis using 84891-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84891-2-RR (AMACR/p504S antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

AMACR/p504S {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 84891-2-RR

Clone No.242456D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

AMACR, p504S, EC:5.1.99.4, Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase

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Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, MCF-7 cells, HepG2 cells, DU 145 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84891-2-RR targets AMACR/p504S in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AMACR/p504S fusion protein Ag8720
• Full Name: alpha-methylacyl-CoA racemase
• Calculated Molecular Weight: 382 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC009471
• Gene Symbol: AMACR
• Gene ID (NCBI): 23600
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UHK6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMACR(Alpha-methyl acyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. A mitochondrial and peroxisomal enzyme catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing and can form a dimer of 70-75 kDa(PMID:21812041). Defects in AMACR cause alpha-methyl acyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).