ALS2 Polyclonal antibody

ALS2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13998-1-AP

Synonyms

ALS2, ALS2CR6, Alsin, ALSJ, IAHSP, KIAA1563, PLSJ



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13998-1-AP targets ALS2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALS2 fusion protein Ag5087
Full Name amyotrophic lateral sclerosis 2 (juvenile)
Calculated Molecular Weight 1657 aa, 184 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC029174
Gene Symbol ALS2
Gene ID (NCBI) 57679
RRIDAB_10858476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ALS2 antibody 13998-1-APDownload protocol
IHC protocol for ALS2 antibody 13998-1-APDownload protocol
IF protocol for ALS2 antibody 13998-1-APDownload protocol
IP protocol for ALS2 antibody 13998-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols