ALPPL2 Recombinant antibody

ALPPL2 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241867A12

Cat no : 84813-1-RR

Synonyms

ALPG, ALP-1, ALP1, Alkaline phosphatase, placental-like, Alkaline phosphatase, germ cell type

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Validation Data Gallery

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  WB analysis of human placenta using 84813-1-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84813-1-RR (ALPPL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 84813-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84813-1-RR (ALPPL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 84813-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84813-1-RR (ALPPL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84813-1-RR is ALPPL2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ALPPL2 fusion protein Ag13360
• Full Name: alkaline phosphatase, placental-like 2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC014139
• Gene Symbol: ALPPL2
• Gene ID (NCBI): 251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALPPL2 (alkaline phosphatase placental-like 2), a member of the human alkaline phosphatase family, could be a potential biomarker for early diagnosis of Pancreatic ductal adenocarcinoma.