ALPL Recombinant antibody

ALPL Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230212D5

Cat no : 82915-2-RR

Synonyms

Alkaline phosphatase, Alkaline phosphatase, tissue-nonspecific isozyme, Alkaline phosphatase tissue nonspecific isozyme, Alkaline phosphatase liver/bone/kidney isozyme, AKP2

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Validation Data Gallery

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  WB analysis of L02 using 82915-2-RR

  L02 cells were subjected to SDS PAGE followed by western blot with 82915-2-RR (ALPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 82915-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ALPL Recombinant antibody (82915-2-RR, Clone:230212D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: L02 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82915-2-RR is ALPL Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ALPL fusion protein Ag1664
• Full Name: alkaline phosphatase, liver/bone/kidney
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC021289
• Gene Symbol: ALPL
• Gene ID (NCBI): 249
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.