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  • KD/KO Validated

ALKBH7 Polyclonal antibody

ALKBH7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15470-1-AP

Synonyms

ABH7, ALKBH7, SPATA11, UNQ6002



Tested Applications

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15470-1-AP targets ALKBH7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALKBH7 fusion protein Ag7772
Full Name alkB, alkylation repair homolog 7 (E. coli)
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC004393
Gene Symbol ALKBH7
Gene ID (NCBI) 84266
RRIDAB_2226855
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALKBH7(Alkylated DNA repair protein alkB homolog 7) is also named as ABH7, SPATA11 and belongs to the alkB family. This protein may be a dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron. Quantitative real-time PCR detectes variable ALKBH7 expression in all 16 tissues examined, with highest expression in pancreas, followed by spleen, prostate, ovary, and placenta(PMID:17979886). The gene encodes a 25 kDa protein with a 20 amino acid signal peptide.

Protocols

Product Specific Protocols
WB protocol for ALKBH7 antibody 15470-1-APDownload protocol
IHC protocol for ALKBH7 antibody 15470-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing.

Authors - Li-Sheng Zhang
  • KD Validated
human,mouseWB

Cell Death Dis

ALKBH7 drives a tissue and sex-specific necrotic cell death response following alkylation-induced damage.

Authors - Jennifer J Jordan
  • KO Validated
humanWB

Cell

A glycolytic metabolite bypasses "two-hit" tumor suppression by BRCA2

Authors - Li Ren Kong