ALKBH7 Polyclonal antibody

• Featured Product
• KD/KO Validated

ALKBH7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15470-1-AP

Synonyms

ABH7, ALKBH7, SPATA11, UNQ6002

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Validation Data Gallery

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  WB analysis of human testis using 15470-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15470-1-AP (ALKBH7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15470-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15470-1-AP (ALKBH7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 15470-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15470-1-AP (ALKBH7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15470-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15470-1-AP (ALKBH7 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below

Product Information

15470-1-AP targets ALKBH7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALKBH7 fusion protein Ag7772
• Full Name: alkB, alkylation repair homolog 7 (E. coli)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC004393
• Gene Symbol: ALKBH7
• Gene ID (NCBI): 84266
• RRID: AB_2226855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALKBH7(Alkylated DNA repair protein alkB homolog 7) is also named as ABH7, SPATA11 and belongs to the alkB family. This protein may be a dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron. Quantitative real-time PCR detectes variable ALKBH7 expression in all 16 tissues examined, with highest expression in pancreas, followed by spleen, prostate, ovary, and placenta(PMID:17979886). The gene encodes a 25 kDa protein with a 20 amino acid signal peptide.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing.; Authors - Li-Sheng Zhang ; KD Validated
human,mouse	WB	Cell Death Dis; ALKBH7 drives a tissue and sex-specific necrotic cell death response following alkylation-induced damage.; Authors - Jennifer J Jordan ; KO Validated
human	WB	Cell; A glycolytic metabolite bypasses "two-hit" tumor suppression by BRCA2; Authors - Li Ren Kong