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  • Featured Product
  • KD/KO Validated

ALKBH5 Recombinant antibody, PBS Only

ALKBH5 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2K9

Cat no : 82083-1-PBS

Synonyms

ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ALKBH5 Recombinant antibody (82083-1-RR)

    WB analysis of HeLa using 82083-1-RR (same clone as 82083-1-PBS)

    WB result of ALKBH5 antibody (82083-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALKBH5 transfected HeLa cells. This data was developed using the same antibody clone with 82083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ALKBH5 Recombinant antibody (82083-1-RR)

    WB analysis using 82083-1-RR (same clone as 82083-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82083-1-RR (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82083-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using ALKBH5 Recombinant antibody (82083-1-RR)

    IF Staining of U-251 using 82083-1-RR (same clone as 82083-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ALKBH5 antibody (82083-1-RR, Clone: 2K9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82083-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82083-1-PBS targets ALKBH5 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ALKBH5 fusion protein Ag10516
Full Name alkB, alkylation repair homolog 5 (E. coli)
Calculated Molecular Weight 458 aa, 51 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC062339
Gene Symbol ALKBH5
Gene ID (NCBI) 54890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.