ALKBH3 Recombinant antibody

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  WB analysis using 84314-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84314-3-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84314-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84314-3-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 84314-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ALKBH3 antibody (84314-3-RR, Clone: 241650C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 84314-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-3-RR, Clone:241650C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of PC-3 using 84314-3-RR

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-3-RR, Clone:241650C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84314-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84314-3-RR against Human ALKBH3 were performed. The affinity constant is 1.42 nM.

ALKBH3 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 84314-3-RR

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

DEPC-1, DEPC1, DEPC 1, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, ABH3

Formulation:  PBS and Azide

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse kidney tissue, mouse heart tissue, rat heart tissue
• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: PC-3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84314-3-RR targets ALKBH3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ALKBH3 fusion protein Ag2982
• Full Name: alkB, alkylation repair homolog 3 (E. coli)
• Calculated Molecular Weight: 286 aa, 33 kDa
• Observed Molecular Weight: 19-25 kDa
• GenBank Accession Number: BC015155
• Gene Symbol: ALKBH3
• Gene ID (NCBI): 221120
• RRID: AB_3671856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96Q83
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.