ALKBH3 Recombinant antibody, PBS Only (Capture)

ALKBH3 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241650F10

Cat no : 84314-2-PBS

Synonyms

DEPC-1, DEPC1, DEPC 1, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, ABH3

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Validation Data Gallery

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  WB analysis using 84314-2-RR (same clone as 84314-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84314-2-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

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  WB analysis using 84314-2-RR (same clone as 84314-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84314-2-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01211-2

  Sandwich ELISA standard curve of MP01211-2, Human ALKBH3 Recombinant Matched Antibody Pair - PBS only. 84314-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2982. 84314-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP01211-2

  The mean ALKBH3 concentration was determined to be 6.80 ng/mL in Jurkat cell extract based on a 1.50 mg/mL extract load.

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  FC experiment of HeLa using 84314-2-RR (same clone as 84314-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-2-RR, Clone:241650F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

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  FC experiment of PC-3 using 84314-2-RR (same clone as 84314-2-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-2-RR, Clone:241650F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01211-1

  Cytometric bead array standard curve of MP01211-1, ALKBH3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84314-2-PBS. Detection antibody: 84314-1-PBS. Standard: Ag2982. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84314-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 84314-2-PBS against Human ALKBH3 was performed. The affinity constant is 0.59 nM.

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  Affinity and Kinetic Characterization of 84314-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84314-2-RR against Human ALKBH3 were performed. The affinity constant is 1.99 nM.

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Product Information

84314-2-PBS targets ALKBH3 as part of a matched antibody pair:

MP01211-1: 84314-2-PBS capture and 84314-1-PBS detection (validated in Cytometric bead array)

MP01211-2: 84314-2-PBS capture and 84314-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ALKBH3 fusion protein Ag2982
• Full Name: alkB, alkylation repair homolog 3 (E. coli)
• Calculated Molecular Weight: 286 aa, 33 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC015155
• Gene Symbol: ALKBH3
• Gene ID (NCBI): 221120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.