ALKBH2 Polyclonal antibody

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  WB analysis using 17809-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17809-1-AP (ALKBH2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

ALKBH2 Polyclonal Antibody for WB, ELISA

Cat No. 17809-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ABH2, ALKBH2, Oxy DC1

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Tested Applications

• Positive WB detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17809-1-AP targets ALKBH2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALKBH2 fusion protein Ag12108
• Full Name: alkB, alkylation repair homolog 2 (E. coli)
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC070489
• Gene Symbol: ALKBH2
• Gene ID (NCBI): 121642
• RRID: AB_3085541
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6NS38
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Escherichia coli AlkB is a DNA repair enzyme that represents the founding member of the AlkB family of non-heme iron-dependent dioxygenases. The human genome encodes nine AlkB homologs termed AlkB homolog 1 through 8 (ALKBH1 through 8) plus a ninth member termed fat mass and obesity associated (FTO). Bacterial AlkB as well as human ALKBH2 and ALKBH3 catalyze the direct reversal of DNA methylation damage through a distinct oxidative demethylation reaction. Importantly, ALKBH2 has been shown to play a role in DNA alkylation damage repair in human, mouse and plant cells. Full-length ALKBH2 has a molecular weight of about 30kDa and ALKBH2 variant encoded by an alternatively spliced transcript with a frameshift deletion of the C-terminal dioxygenase domain has a molecular weight of about 25kDa (PMID:22079122).