ALKBH1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ALKBH1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27973-1-AP

Synonyms

ABH, ABH1, alkB, ALKBH, ALKBH1, DNA lyase ABH1, hABH

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Validation Data Gallery

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  WB analysis of A549 using 27973-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27973-1-AP (ALKBH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

27973-1-AP targets ALKBH1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALKBH1 fusion protein Ag27520
• Full Name: alkB, alkylation repair homolog 1 (E. coli)
• Calculated Molecular Weight: 389 aa, 44 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC025787
• Gene Symbol: ALKBH1
• Gene ID (NCBI): 8846
• RRID: AB_2881025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Two RNA m6A demethylases, FTO and ALKBH5, have been identified since 2011, with both capable of removing the methyl group of m6A on poly-adenylated RNA through an iron-dependent oxidative demethylation mechanism. ALKBH1 as a RNA demethylase that mediates removal of the methyl group from N1-methyladenosine (m1A) in tRNA. ALKBH1 can tune translation initiation and elongation by regulating the cellular levels of tRNAiMet and the association of other ALKBH1 target tRNAs to polysomes, thereby affecting protein synthesis.

Publications

Species	Application	Title
human	WB	Cell Discov; ALKBH3-dependent m1A demethylation of Aurora A mRNA inhibits ciliogenesis.; Authors - Wenjun Kuang ; KD Validated