ALDOB Polyclonal antibody

• Featured Product
• KD/KO Validated

ALDOB Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

34

Cat no : 18065-1-AP

Synonyms

Liver-type aldolase, Fructose-bisphosphate aldolase B, EC:4.1.2.13, ALDB

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Validation Data Gallery

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  WB analysis using 18065-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 18065-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18065-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18065-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 18065-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 18065-1-AP

  IP result of anti-ALDOB (IP:18065-1-AP, 4ug; Detection:18065-1-AP 1:500) with mouse brain tissue lysate 2400 ug.

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  IP experiment of mouse kidney using 18065-1-AP

  IP result of anti-ALDOB (IP:18065-1-AP, 4ug; Detection:18065-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human liver cancer using 18065-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18065-1-AP (ALDOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 18065-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18065-1-AP (ALDOB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 18065-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18065-1-AP (ALDOB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18065-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18065-1-AP (ALDOB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue, mouse brain tissue, Raji cells, mouse skeletal muscle tissue, rat kidney tissue
• Positive IP detected in: mouse brain tissue, mouse kidney tissue
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 31 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• IP: See 3 publications below

Product Information

18065-1-AP targets ALDOB in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDOB fusion protein Ag12695
• Full Name: aldolase B, fructose-bisphosphate
• Calculated Molecular Weight: 316 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC029399
• Gene Symbol: ALDOB
• Gene ID (NCBI): 229
• RRID: AB_2273968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A (ALDOA), B (ALDOB), and C (ALDOC). Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody may detect ALDOA.

Publications

Species	Application	Title
mouse	WB	Nature; Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.; Authors - Chen-Song Zhang ; KD Validated
human	IF	Cancer Cell; Proteogenomic characterization identifies clinically relevant subgroups of intrahepatic cholangiocarcinoma.; Authors - Liangqing Dong
human	WB	Nat Commun; O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates leucine and glucose availability to regulate mTORC1 and the metabolic fate of leucine.; Authors - Kibum Kim
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
human	WB,IP	Free Radic Biol Med; SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.; Authors - Yuanzhen Ma