ALDOA Recombinant antibody

• Featured Product
• KD/KO Validated

ALDOA Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Pig, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F4

Cat no : 80837-1-RR

Synonyms

Lung cancer antigen NY-LU-1, Fructose-bisphosphate aldolase A, EC:4.1.2.13, Aldolase A, ALDA

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Validation Data Gallery

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  WB analysis using 80837-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80837-1-RR (ALDOA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 80837-1-RR

  WB result of ALDOA antibody (80837-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDOA transfected HEK-293 cells.

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  IF Staining of HeLa using 80837-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ALDOA antibody (80837-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 80837-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ALDOA antibody (80837-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 80837-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80837-1-RR against Human ALDOA were performed. The affinity constant is 1.34 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells, HSC-T6 cells, pig liver tissue, pig skeletal muscle tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80837-1-RR targets ALDOA in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig, rat samples.

• Tested Reactivity: Human, Mouse, Pig, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ALDOA fusion protein Ag1726
• Full Name: aldolase A, fructose-bisphosphate
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC016800
• Gene Symbol: ALDOA
• Gene ID (NCBI): 226
• RRID: AB_2918914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).