ALDOA Monoclonal antibody, PBS Only

ALDOA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D6C2

Cat no : 67453-1-PBS

Synonyms

ALDA, ALDOA, Lung cancer antigen NY LU 1, Muscle type aldolase

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Validation Data Gallery

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  WB analysis using 67453-1-Ig (same clone as 67453-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67453-1-Ig (ALDOA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67453-1-Ig (same clone as 67453-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67453-1-Ig (ALDOA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67453-1-Ig (same clone as 67453-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67453-1-Ig (ALDOA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67453-1-Ig (same clone as 67453-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67453-1-Ig (ALDOA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67453-1-Ig (same clone as 67453-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDOA antibody (67453-1-Ig, Clone: 1D6C2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

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Product Information

67453-1-PBS targets ALDOA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALDOA fusion protein Ag17952
• Full Name: aldolase A, fructose-bisphosphate
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC016800
• Gene Symbol: ALDOA
• Gene ID (NCBI): 226
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).