ALDH7A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ALDH7A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 10368-1-AP

Synonyms

ALDH7A1, Alpha AASA dehydrogenase, Antiquitin 1, ATQ1, Betaine aldehyde dehydrogenase, EPD, P6c dehydrogenase, PDE

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Validation Data Gallery

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  WB analysis using 10368-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10368-1-AP (ALDH7A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10368-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10368-1-AP (ALDH7A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10368-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10368-1-AP (ALDH7A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10368-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10368-1-AP (ALDH7A1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10368-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10368-1-AP (ALDH7A1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse kidney tissue, mouse liver tissue, HeLa cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10368-1-AP targets ALDH7A1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH7A1 fusion protein Ag0383
• Full Name: aldehyde dehydrogenase 7 family, member A1
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC002515
• Gene Symbol: ALDH7A1
• Gene ID (NCBI): 501
• RRID: AB_2242476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH7A1(Alpha-aminoadipic semialdehyde dehydrogenase) is also named as ATQ1 and belongs to the aldehyde dehydrogenase family. It plays a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This protein has 4 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse, rat	WB	Arch Toxicol; Protective effects of dioscin against alcohol-induced liver injury.; Authors - Xu Tingting T
rat	WB	Food Funct; Correction: Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.; Authors - Deshi Dong
human	IHC,WB,IF	Exp Cell Res; EPS8 supports pancreatic cancer growth by inhibiting BMI1 mediated proteasomal degradation of ALDH7A1.; Authors - Mingzhu Tan ; KD Validated
rat	WB	PLoS One; Metabolomic mechanisms of gypenoside against liver fibrosis in rats: An integrative analysis of proteomics and metabolomics data.; Authors - Ya-Nan Song
rat	WB	Food Funct; Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.; Authors - Deshi Dong
		J Toxicol Sci; Mechanisms of CCl4-induced liver fibrosis with combined transcriptomic and proteomic analysis.; Authors - Shu Dong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (12-04-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse liver, mouse hippocampus / HepG2

ALDH7A1 Antibody Western Blot, validation (1:1000 dilution) in mouse liver, mouse hippocampus / HepG2 (Cat no:10368-1-AP)