ALDH5A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ALDH5A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 17319-1-AP

Synonyms

ALDH5A1, SSADH, SSDH

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Validation Data Gallery

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  WB analysis using 17319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17319-1-AP (ALDH5A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17319-1-AP

  WB result of ALDH5A1 antibody (17319-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH5A1 transfected HepG2 cells.

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  IHC staining of human liver cancer using 17319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17319-1-AP (ALDH5A1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 17319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17319-1-AP (ALDH5A1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 17319-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

17319-1-AP targets ALDH5A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH5A1 fusion protein Ag11293
• Full Name: aldehyde dehydrogenase 5 family, member A1
• Calculated Molecular Weight: 535 aa, 57 kDa
• Observed Molecular Weight: 51-59 kDa
• GenBank Accession Number: BC034321
• Gene Symbol: ALDH5A1
• Gene ID (NCBI): 7915
• RRID: AB_2224478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Ethnopharmacol; Multi-omics and chemical profiling approaches to understand the material foundation and pharmacological mechanism of Sophorae Tonkinensis Radix et Rhizome-induced liver injury in mice; Authors - Si-Wei Rao
human	IHC	Cancer Biol Ther; Decreased expression of ALDH5A1 predicts prognosis in patients with ovarian cancer.; Authors - Xun Tian
mouse	WB	Neuropharmacology; A role of GABAA receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine; Authors - Xiao-Hui Tang
human,mouse	WB,IF	Aging (Albany NY); Mitochondrial-related genes PDK2, CHDH, and ALDH5A1 served as a diagnostic signature and correlated with immune cell infiltration in ulcerative colitis; Authors - Qian Yang
mouse	WB	Ecotoxicol Environ Saf; 1,2-Dichloroethane causes anxiety and cognitive dysfunction in mice by disturbing GABA metabolism and inhibiting the cAMP-PKA-CREB signaling pathway; Authors - Yuchen Qin