ALDH4A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ALDH4A1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11604-1-AP

Synonyms

ALDH4, ALDH4A1, P5C dehydrogenase, P5CD, P5CDh, P5CDhL, P5CDhS

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Validation Data Gallery

• 

  WB analysis of human heart using 11604-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11604-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 11604-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11604-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11604-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11604-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11604-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11604-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11604-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 11604-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 11604-1-AP

  IP result of anti-ALDH4A1 (IP:11604-1-AP, 4ug; Detection:11604-1-AP 1:800) with mouse skeletal muscle tissue lysate 3600ug.

• 

  IHC staining of human liver cancer using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human heart tissue, HepG2 cells, human brain tissue, human liver tissue, human skeletal muscle tissue, K-562 cells, mouse skeletal muscle tissue, mouse small intestine tissue, rat heart tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human liver cancer tissue, human liver tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

11604-1-AP targets ALDH4A1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH4A1 fusion protein Ag2147
• Full Name: aldehyde dehydrogenase 4 family, member A1
• Calculated Molecular Weight: 563 aa, 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC023600
• Gene Symbol: ALDH4A1
• Gene ID (NCBI): 8659
• RRID: AB_2258046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH4A1(Aldehyde dehydrogenase family 4 member A1) is also named as ALDH4, P5CDH,P5C dehydrogenase and belongs to the aldehyde dehydrogenase family. P5CDH is a mitochondrial matrix NAD(+)-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate.Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2).

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.; Authors - Liu Wei W ; KD Validated
human	WB	Sci Rep; Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.; Authors - Bobby Jones
human,mouse	WB,IHC	J Exp Clin Cancer Res; Pharmacological targeting PTK6 inhibits the JAK2/STAT3 sustained stemness and reverses chemoresistance of colorectal cancer.; Authors - Chaoqun Liu
mouse	WB	Nat Metab; Inhibition of the proline metabolism rate-limiting enzyme P5CS allows proliferation of glutamine-restricted cancer cells; Authors - Samantha J Linder ; KD Validated