ALDH1L1 Monoclonal antibody, PBS Only

ALDH1L1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C1A3

Cat no : 68018-1-PBS

Synonyms

10 FTHFDH, ALDH1L1, DKFZp781N0997, FTHFD

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Validation Data Gallery

• 

  WB analysis of human milk using 68018-1-Ig (same clone as 68018-1-PBS)

  human milk were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 68018-1-Ig (same clone as 68018-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 68018-1-Ig (same clone as 68018-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 68018-1-Ig (same clone as 68018-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68018-1-Ig (same clone as 68018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68018-1-Ig (same clone as 68018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:32000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68018-1-PBS targets ALDH1L1 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse, pig samples.

• Tested Reactivity: Human, rat, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALDH1L1 fusion protein Ag13190
• Full Name: aldehyde dehydrogenase 1 family, member L1
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC027241
• Gene Symbol: ALDH1L1
• Gene ID (NCBI): 10840
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.