ALDH1A3 Polyclonal antibody

ALDH1A3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29373-1-AP

Synonyms

Aldehyde dehydrogenase 6, ALDH1A3, ALDH1A6, ALDH6, RALDH 3, RALDH3, Retinaldehyde dehydrogenase 3

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Validation Data Gallery

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  WB analysis using 29373-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29373-1-AP (ALDH1A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 29373-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29373-1-AP (ALDH1A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 29373-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29373-1-AP (ALDH1A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

29373-1-AP targets ALDH1A3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH1A3 fusion protein Ag31100
• Full Name: aldehyde dehydrogenase 1 family, member A3
• Calculated Molecular Weight: 512 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC069274
• Gene Symbol: ALDH1A3
• Gene ID (NCBI): 220
• RRID: AB_2918287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH1A3 is the predominant ALDH isozyme that participates in various physiological processes in human cells. Growing evidence has shown that ALDH1A3 exhibits high activity in cancer stem cells (CSCs) and influences a diverse range of biological characteristics within these cells. (PMID: 30958800,PMID: 32612951)

Publications

Species	Application	Title
human	WB,IHC	J Exp Clin Cancer Res; Tumor associated macrophages-derived exosomes facilitate hepatocellular carcinoma malignance by transferring lncMMPA to tumor cells and activating glycolysis pathway; Authors - Minghao Xu