CoraLite®594 Anti-Human ALCAM (3A6) Mouse IgG2a Recombinant Antibody

ALCAM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3A6

Cat no : CL594-65611

Synonyms

MEMD, CD166, 3A6

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Validation Data Gallery

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  FC experiment of human PBMCs using CL594-65611

  1x10^6 PHA treated human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human ALCAM Mouse IgG2a Recombinant Antibody (CL594-65611, Clone: 3A6) (red) or CoraLite®594 Mouse IgG2a Isotype Control (CL594-65208, Clone: C1.18.4) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human ALCAM Mouse IgG2a Recombinant Antibody (CL594-65611, Clone: 3A6) (black, dash). Cells were not fixed.

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Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65611 targets ALCAM in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Cultured human thymic epithelial cells
• Full Name: activated leukocyte cell adhesion molecule
• Calculated Molecular Weight: 105 kDa
• GenBank Accession Number: BC057809
• Gene Symbol: ALCAM
• Gene ID (NCBI): 214
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).