ALCAM Recombinant antibody

ALCAM Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241945A4

Cat no : 84528-1-RR

Synonyms

Protein DM-GRASP, CD166 antigen, CD166, BEN, Activated leukocyte cell adhesion molecule

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Validation Data Gallery

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  WB analysis of mouse lung using 84528-1-RR

  mouse lung tissue was subjected to SDS PAGE followed by western blot with 84528-1-RR (ALCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 84528-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 84528-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84528-1-RR targets ALCAM in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: activated leukocyte cell adhesion molecule
• Calculated Molecular Weight: 65kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: NM_009655.3
• Gene Symbol: Alcam
• Gene ID (NCBI): 11658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).