Mouse ALCAM Recombinant antibody, PBS Only (Capture)

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  WB analysis of mouse lung using 84528-1-RR (same clone as 84528-1-PBS)

  mouse lung tissue was subjected to SDS PAGE followed by western blot with 84528-1-RR (ALCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01379-1

  Sandwich ELISA standard curve of MP01379-1, Mouse ALCAM Recombinant Matched Antibody Pair - PBS only. 84528-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1524. 84528-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP01379-1

  Serum of six mice was measured. The ALCAM concentration of detected samples was determined to be 43,380.5 pg/mL with a range of 28,091.3-50,380.4 pg/mL.

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  Sample test of MP01379-1

  The mean ALCAM concentration was determined to be 888,916.9 pg/mL in mouse liver cell extract based on a 3.7 mg/mL extract load and 518,424.5 pg/mL in mouse brain cell extract based on a 2.0 mg/mL extract load.

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  IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01379-1

  Cytometric bead array standard curve of MP01379-1, MOUSE ALCAM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84528-1-PBS. Detection antibody: 84528-3-PBS. Standard: Eg1524. Range: 0.781-100 ng/mL.

ALCAM Uni-rAb^TM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84528-1-PBS

Clone No.241945A4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Protein DM-GRASP, CD166 antigen, CD166, BEN, Activated leukocyte cell adhesion molecule

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Product Information

84528-1-PBS targets ALCAM as part of a matched antibody pair:

MP01379-1: 84528-1-PBS capture and 84528-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: activated leukocyte cell adhesion molecule
• Calculated Molecular Weight: 65kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: NM_009655.3
• Gene Symbol: Alcam
• Gene ID (NCBI): 11658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q61490
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).